Application
Crystal violet is commonly used in the Gram stain and its variants; and to stain amyloid, bacterial components, and vascular plant tissues. It is also used for the staining of plant chromosomes.Crystal violet is also suitable for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal Violet also helps in the colorimetric measurement of cell viability.
General description
Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It is a hexa-N-methylated triaminotriphenylmethane with a lipophilic cation. It possesses a slightly nonplanar conjugated system.
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