Crystal Violet, for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)

Code: 61135-100G D2-231

Application

Crystal violet is commonly used in the Gram stain and its variants; and to stain amyloid, bacterial components, and vascular plant tissues. It is also used for th...


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€83.10 100G
€102.21 inc. VAT

Application

Crystal violet is commonly used in the Gram stain and its variants; and to stain amyloid, bacterial components, and vascular plant tissues. It is also used for the staining of plant chromosomes.Crystal violet is also suitable for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal Violet also helps in the colorimetric measurement of cell viability.

General description

Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It is a hexa-N-methylated triaminotriphenylmethane with a lipophilic cation. It possesses a slightly nonplanar conjugated system.

ε (extinction coefficient)≥1750 at 585-595 nm in water
antibiotic activity spectrumfungi
application(s)diagnostic assay manufacturinghematologyhistology
density1.190 g/cm3
formpowder
gradefor microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)
InChI keyZXJXZNDDNMQXFV-UHFFFAOYSA-M
InChI1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
mode of actioncell membrane | interferes, enzyme | inhibits
mp205 °C (dec.) (lit.)
Quality Level200
SMILES string[Cl-].CN(C)c1ccc(cc1)C(c2ccc(cc2)N(C)C)=C3/C=CC(C=C3)=[N+](/C)C
storage temp.room temp
suitabilitysuitable for microscopy (Bact., Bot., Hist., Vit.)
visual transition interval0.1-2.0, yellow-green to blue-violet
Cas Number548-62-9
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